Systems and methods for epigenetic sequencing

David Weitz (Inventor), Assaf Rotem (Inventor), Oren Ram (Inventor), Bradley E Bernstein (Inventor)

Research output: Patent


The present invention generally relates to microfluidics and/or epigenetic sequencing. In one set of embodiments, cells contained within a plurality of microfluidic droplets are lysed and the DNA (e.g., from nucleosomes) within the droplets are labeled, e.g., with adapters containing an identification sequence. The adapters may also contain other sequences, e.g., restriction sites, primer sites, etc., to assist with later analysis. After labeling with adapters, the DNA from the different cells may be combined and analyzed, e.g., to determine epigenetic information about the cells. For example, the DNA may be separated on the basis of certain modifications (e.g., methylation), and the DNA from the separated nucleosomes may be sequenced using techniques such as chromatin immunoprecipitation (“CUP”). In some cases, the DNA sequences may also be aligned with genomes, e.g., to determine which portions of the genome were epigenetically modified, e.g., via methylation.

Original languageAmerican English
Patent numberUS2021355535
IPCC12Q 1/ 6869 A I
Priority date25/05/21
StatePublished - 18 Nov 2021


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