Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain; Chethan D. Shanthamurthy; Shani Leviatan Ben-Arye; Sharon Yehuda; Sharvani S. Nandikol; Hirekodathakallu V. Thulasiram; Vered Padler-Karavani; Raghavendra Kikkeri

doi:10.1039/d1cc00964h

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain
, Chethan D. Shanthamurthy
, Shani Leviatan Ben-Arye
, Sharon Yehuda
, Sharvani S. Nandikol
, Hirekodathakallu V. Thulasiram
, Vered Padler-Karavani
, Raghavendra Kikkeri

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF₁₆₅, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO₃⁻)-IdoA(2-O-SO₃⁻) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF₁₆₅growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF₁₆₅andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

Original language	English GB
Pages (from-to)	3516-3519
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	28
DOIs	https://doi.org/10.1039/d1cc00964h
State	Published - 11 Apr 2021

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc00964h

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title = "Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis",

abstract = "We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.",

author = "Prashant Jain and Shanthamurthy, \{Chethan D.\} and \{Leviatan Ben-Arye\}, Shani and Sharon Yehuda and Nandikol, \{Sharvani S.\} and Thulasiram, \{Hirekodathakallu V.\} and Vered Padler-Karavani and Raghavendra Kikkeri",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = apr,

day = "11",

doi = "10.1039/d1cc00964h",

language = "الإنجليزيّة",

volume = "57",

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TY - JOUR

T1 - Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

AU - Jain, Prashant

AU - Shanthamurthy, Chethan D.

AU - Leviatan Ben-Arye, Shani

AU - Yehuda, Sharon

AU - Nandikol, Sharvani S.

AU - Thulasiram, Hirekodathakallu V.

AU - Padler-Karavani, Vered

AU - Kikkeri, Raghavendra

PY - 2021/4/11

Y1 - 2021/4/11

N2 - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

AB - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

UR - https://www.scopus.com/pages/publications/85103725519

U2 - 10.1039/d1cc00964h

DO - 10.1039/d1cc00964h

M3 - مقالة

C2 - 33704312

SN - 1359-7345

VL - 57

SP - 3516

EP - 3519

JO - Chemical Communications

JF - Chemical Communications

IS - 28

ER -

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Abstract

ASJC Scopus subject areas

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