Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain; Chethan D. Shanthamurthy; Shani Leviatan Ben-Arye; Sharon Yehuda; Sharvani S. Nandikol; Hirekodathakallu V. Thulasiram; Vered Padler-Karavani; Raghavendra Kikkeri

doi:10.1039/d1cc00964h

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu V. Thulasiram, Vered Padler-Karavani, Raghavendra Kikkeri

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF₁₆₅, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO₃⁻)-IdoA(2-O-SO₃⁻) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF₁₆₅growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF₁₆₅andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

Original language	English
Pages (from-to)	3516-3519
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	28
DOIs	https://doi.org/10.1039/d1cc00964h
State	Published - 11 Apr 2021

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

Access to Document

10.1039/d1cc00964h

Cite this

@article{c6a212158bfe47f3b46e2695afc615a3,

title = "Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis",

abstract = "We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.",

author = "Prashant Jain and Shanthamurthy, {Chethan D.} and {Leviatan Ben-Arye}, Shani and Sharon Yehuda and Nandikol, {Sharvani S.} and Thulasiram, {Hirekodathakallu V.} and Vered Padler-Karavani and Raghavendra Kikkeri",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = apr,

day = "11",

doi = "10.1039/d1cc00964h",

language = "الإنجليزيّة",

volume = "57",

pages = "3516--3519",

number = "28",

}

TY - JOUR

T1 - Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

AU - Jain, Prashant

AU - Shanthamurthy, Chethan D.

AU - Leviatan Ben-Arye, Shani

AU - Yehuda, Sharon

AU - Nandikol, Sharvani S.

AU - Thulasiram, Hirekodathakallu V.

AU - Padler-Karavani, Vered

AU - Kikkeri, Raghavendra

PY - 2021/4/11

Y1 - 2021/4/11

N2 - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

AB - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

UR - http://www.scopus.com/inward/record.url?scp=85103725519&partnerID=8YFLogxK

U2 - 10.1039/d1cc00964h

DO - 10.1039/d1cc00964h

M3 - مقالة

C2 - 33704312

SN - 1359-7345

VL - 57

SP - 3516

EP - 3519

JO - Chemical Communications

JF - Chemical Communications

IS - 28

ER -

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this