Synthesis and Evaluation of Indole-Based Autoinducers on Quorum Sensing in Vibrio cholerae

Lara Holoidovsky; Michael M. Meijler

doi:10.1021/acsinfecdis.9b00409

Synthesis and Evaluation of Indole-Based Autoinducers on Quorum Sensing in Vibrio cholerae

Lara Holoidovsky, Michael M. Meijler

Department of Chemistry

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Vibrio cholerae (V. cholerae) uses the autoinducer CAI-1 (cholera autoinducer 1) and several linked quorum sensing systems in order to efficiently sense its ever-changing environment and optimally coordinate population-wide gene expression. Indole has been reported as an important signal that is sensed by V. cholerae, and here, we report the synthesis and evaluation of a focused library of synthetic indole-CAI-1 derivatives as tools to probe quorum sensing (QS) in this human pathogen. Our results show interesting and diverging effects for several conjugates, as compared to CAI-1, on virulence factor production and biofilm formation.

Original language	American English
Pages (from-to)	572-576
Number of pages	5
Journal	ACS Infectious Diseases
Volume	6
Issue number	4
DOIs	https://doi.org/10.1021/acsinfecdis.9b00409
State	Published - 10 Apr 2020

Keywords

CAI-1
CqsS
Vibrio cholerae
biofilm
cholera toxin
indole
quorum sensing

All Science Journal Classification (ASJC) codes

Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsinfecdis.9b00409

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@article{b35e8ef4af00435cb8fb52be23b2ccef,

title = "Synthesis and Evaluation of Indole-Based Autoinducers on Quorum Sensing in Vibrio cholerae",

abstract = "Vibrio cholerae (V. cholerae) uses the autoinducer CAI-1 (cholera autoinducer 1) and several linked quorum sensing systems in order to efficiently sense its ever-changing environment and optimally coordinate population-wide gene expression. Indole has been reported as an important signal that is sensed by V. cholerae, and here, we report the synthesis and evaluation of a focused library of synthetic indole-CAI-1 derivatives as tools to probe quorum sensing (QS) in this human pathogen. Our results show interesting and diverging effects for several conjugates, as compared to CAI-1, on virulence factor production and biofilm formation.",

keywords = "CAI-1, CqsS, Vibrio cholerae, biofilm, cholera toxin, indole, quorum sensing",

author = "Lara Holoidovsky and Meijler, {Michael M.}",

note = "Funding Information: We would like to thank Bonnie L. Bassler for providing Vibrio cholerae strains and helpful discussions regarding the V. cholerae assays. The research was supported by grants from the Israel Science Foundation (Personal Grant 1667/15) and the European Research Council (Starting Grant 240356). Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

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doi = "10.1021/acsinfecdis.9b00409",

language = "American English",

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T1 - Synthesis and Evaluation of Indole-Based Autoinducers on Quorum Sensing in Vibrio cholerae

AU - Holoidovsky, Lara

AU - Meijler, Michael M.

N1 - Funding Information: We would like to thank Bonnie L. Bassler for providing Vibrio cholerae strains and helpful discussions regarding the V. cholerae assays. The research was supported by grants from the Israel Science Foundation (Personal Grant 1667/15) and the European Research Council (Starting Grant 240356). Publisher Copyright: © 2020 American Chemical Society.

PY - 2020/4/10

Y1 - 2020/4/10

N2 - Vibrio cholerae (V. cholerae) uses the autoinducer CAI-1 (cholera autoinducer 1) and several linked quorum sensing systems in order to efficiently sense its ever-changing environment and optimally coordinate population-wide gene expression. Indole has been reported as an important signal that is sensed by V. cholerae, and here, we report the synthesis and evaluation of a focused library of synthetic indole-CAI-1 derivatives as tools to probe quorum sensing (QS) in this human pathogen. Our results show interesting and diverging effects for several conjugates, as compared to CAI-1, on virulence factor production and biofilm formation.

AB - Vibrio cholerae (V. cholerae) uses the autoinducer CAI-1 (cholera autoinducer 1) and several linked quorum sensing systems in order to efficiently sense its ever-changing environment and optimally coordinate population-wide gene expression. Indole has been reported as an important signal that is sensed by V. cholerae, and here, we report the synthesis and evaluation of a focused library of synthetic indole-CAI-1 derivatives as tools to probe quorum sensing (QS) in this human pathogen. Our results show interesting and diverging effects for several conjugates, as compared to CAI-1, on virulence factor production and biofilm formation.

KW - CAI-1

KW - CqsS

KW - Vibrio cholerae

KW - biofilm

KW - cholera toxin

KW - indole

KW - quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=85083542465&partnerID=8YFLogxK

U2 - 10.1021/acsinfecdis.9b00409

DO - 10.1021/acsinfecdis.9b00409

M3 - Article

C2 - 32182033

SN - 2373-8227

VL - 6

SP - 572

EP - 576

JO - ACS Infectious Diseases

JF - ACS Infectious Diseases

IS - 4

ER -

Synthesis and Evaluation of Indole-Based Autoinducers on Quorum Sensing in Vibrio cholerae

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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