Synthesis and evaluation of a tag-free photoactive phospho-ceramide analogue-1 (PCERA-1) probe to study immunomodulation in macrophages

Rambabu Dandela; Roi Mashiach; Raju Adepu; Rachel Gregor; Muhammad Athamna; Efrat Zecharia; Orna Ernst; Tsaffrir Zor; Michael M. Meijler

doi:10.1039/c6cc09849e

Synthesis and evaluation of a tag-free photoactive phospho-ceramide analogue-1 (PCERA-1) probe to study immunomodulation in macrophages

Rambabu Dandela, Roi Mashiach, Raju Adepu, Rachel Gregor, Muhammad Athamna, Efrat Zecharia, Orna Ernst, Tsaffrir Zor, Michael M. Meijler

Ben-Gurion University of the Negev, Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Phospho-ceramide analogue-1 (PCERA-1), a synthetic analogue of ceramide-1-phosphate (C1P), has been previously shown to act as a potent modulator of macrophage activity and inflammation. We have developed an efficient synthesis of PCERA-1 from readily available starting materials, and designed and prepared derivatives of this analogue, including a photoaffinity probe to tag and identify putative proteins that bind PCERA-1.

Original language	American English
Pages (from-to)	3842-3845
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	27
DOIs	https://doi.org/10.1039/c6cc09849e
State	Published - 1 Jan 2017

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c6cc09849e

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abstract = "Phospho-ceramide analogue-1 (PCERA-1), a synthetic analogue of ceramide-1-phosphate (C1P), has been previously shown to act as a potent modulator of macrophage activity and inflammation. We have developed an efficient synthesis of PCERA-1 from readily available starting materials, and designed and prepared derivatives of this analogue, including a photoaffinity probe to tag and identify putative proteins that bind PCERA-1.",

author = "Rambabu Dandela and Roi Mashiach and Raju Adepu and Rachel Gregor and Muhammad Athamna and Efrat Zecharia and Orna Ernst and Tsaffrir Zor and Meijler, {Michael M.}",

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AU - Dandela, Rambabu

AU - Mashiach, Roi

AU - Adepu, Raju

AU - Gregor, Rachel

AU - Athamna, Muhammad

AU - Zecharia, Efrat

AU - Ernst, Orna

AU - Zor, Tsaffrir

AU - Meijler, Michael M.

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AB - Phospho-ceramide analogue-1 (PCERA-1), a synthetic analogue of ceramide-1-phosphate (C1P), has been previously shown to act as a potent modulator of macrophage activity and inflammation. We have developed an efficient synthesis of PCERA-1 from readily available starting materials, and designed and prepared derivatives of this analogue, including a photoaffinity probe to tag and identify putative proteins that bind PCERA-1.

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DO - 10.1039/c6cc09849e

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Synthesis and evaluation of a tag-free photoactive phospho-ceramide analogue-1 (PCERA-1) probe to study immunomodulation in macrophages

Abstract

All Science Journal Classification (ASJC) codes

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