Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Guy Mann; Gandhesiri Satish; Prasad Sulkshane; Shaswati Mandal; Michael H. Glickman; Ashraf Brik

doi:https://doi.org/10.1039/d1cc04045f

Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Guy Mann, Gandhesiri Satish, Prasad Sulkshane, Shaswati Mandal, Michael H. Glickman, Ashraf Brik

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.

Original language	American English
Pages (from-to)	9438-9441
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	74
DOIs	https://doi.org/10.1039/d1cc04045f
State	Published - 21 Sep 2021

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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https://doi.org/10.1039/d1cc04045f

Cite this

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abstract = "Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.",

author = "Guy Mann and Gandhesiri Satish and Prasad Sulkshane and Shaswati Mandal and Glickman, {Michael H.} and Ashraf Brik",

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AU - Satish, Gandhesiri

AU - Sulkshane, Prasad

AU - Mandal, Shaswati

AU - Glickman, Michael H.

AU - Brik, Ashraf

PY - 2021/9/21

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AB - Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.

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DO - https://doi.org/10.1039/d1cc04045f

M3 - Article

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EP - 9441

JO - Chemical Communications

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Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Abstract

All Science Journal Classification (ASJC) codes

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