Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy

Michael L. Buttolph, Menansili A. Mejooli, Pavel Sidorenko, Chi Yong Eom, Chris B. Schaffer, Frank W. Wise

Research output: Contribution to journalArticlepeer-review


Two-photon fluorescence microscopy is a nonlinear imaging modality frequently used in deep-tissue imaging applications. A tunable-wavelength multicolor short-pulse source is usually required to excite fluorophores with a wide range of excitation wavelengths. This need is most typically met by solid-state lasers, which are bulky, expensive, and complicated systems. Here, we demonstrate a compact, robust fiber system that generates naturally synchronized femtosecond pulses at 1050 nm and 1200 nm by using a combination of gain-managed and Raman amplification. We image the brain of a mouse and view the blood vessels, neurons, and other cell-like structures using simultaneous degenerate and nondegenerate excitation.

Original languageEnglish
Pages (from-to)2496-2507
Number of pages12
JournalBiomedical Optics Express
Issue number4
StatePublished - Apr 2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Biotechnology


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