@inbook{cccd843abc9a46bbbfe36d191959a90b,
title = "Synchronization to Visualization: Dissecting Myogenesis and Regeneration Using Correlative Light and Electron Microscopy (CLEM)",
abstract = "Skeletal muscle regeneration is a complex, dynamic process primarily driven by satellite cells located within the muscle tissue. Gaining a deeper understanding of regeneration necessitates high-resolution information on defined stages during the regeneration process. This chapter presents a method that pairs the robust differentiation of primary muscle cells with correlative light and electron microscopy (CLEM) for a detailed study of muscle regeneration. By inhibiting extracellular signal-regulated kinases 1 and 2 (ERK1/2) in primary myoblasts, differentiation, and formation of myotubes are induced in under 24 h post-induction, providing a synchronized platform perfect for on-section CLEM. As a result, capturing ultrastructural details and rare cellular events becomes more precise and efficient. This chapter lays out a comprehensive guide for sample preparation and the principles of data correlation, showcasing how this method can overcome previous challenges to shed light on the molecular dynamics of skeletal muscle regeneration, which is essential for deciphering the complexities of muscle repair and related pathologies.",
author = "Suman Khan and Nadav Scher and Ori Avinoam",
note = "The authors acknowledge funding from the European Research Council (ERC StG # 851080), and the David Barton Centre for Research on the Chemistry of Life. OA is the incumbent of the Miriam Berman Presidential Development Chair.",
year = "2023",
month = nov,
day = "30",
doi = "10.1007/978-3-030-85569-7_5-1",
language = "الإنجليزيّة",
isbn = "978-3-030-85569-7",
series = "Reference Series in Biomedical Engineering",
pages = "1--16",
editor = "Andreas Walter and Paul Slezak and Ralph Mueller and Greet Kerckhofs and Baubak Bajoghli",
booktitle = "Bioimaging in Tissue Engineering and Regeneration: Advanced Microscopy and Preclinical Imaging",
}