Synapsin E-domain is essential for α-synuclein function

Alexandra Stavsky; Leonardo A. Parra-Rivas; Shani Tal; Jen Riba; Kayalvizhi Madhivanan; Subhojit Roy; Daniel Gitler

doi:10.7554/eLife.89687

Synapsin E-domain is essential for α-synuclein function

Alexandra Stavsky, Leonardo A. Parra-Rivas, Shani Tal, Jen Riba, Kayalvizhi Madhivanan, Subhojit Roy, Daniel Gitler

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here, we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at synapses of cultured mouse hippocampal neurons. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.

Original language	American English
Journal	eLife
Volume	12
DOIs	https://doi.org/10.7554/eLife.89687
State	Published - 7 May 2024

Keywords

alpha-synuclein
cell biology
mouse
neuroscience
presynaptic
synapsin
synaptic terminals
synaptic transmission
synaptic vesicles

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.7554/eLife.89687

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title = "Synapsin E-domain is essential for α-synuclein function",

abstract = "The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here, we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at synapses of cultured mouse hippocampal neurons. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.",

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AU - Stavsky, Alexandra

AU - Parra-Rivas, Leonardo A.

AU - Tal, Shani

AU - Riba, Jen

AU - Madhivanan, Kayalvizhi

AU - Roy, Subhojit

AU - Gitler, Daniel

PY - 2024/5/7

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AB - The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here, we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at synapses of cultured mouse hippocampal neurons. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.

KW - alpha-synuclein

KW - cell biology

KW - mouse

KW - neuroscience

KW - presynaptic

KW - synapsin

KW - synaptic terminals

KW - synaptic transmission

KW - synaptic vesicles

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Synapsin E-domain is essential for α-synuclein function

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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