Superresolution saturated structured illumination microscopy system: Theoretical aspects and real life

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Many strategies for improving both axial and lateral resolutions are based on a priori information about the input signal and lead on a numerical aperture improvement. But all of them are still limited by the wave nature of light. By using fluorescence technique one theoretically can reach unlimited resolution. In this talk we present simulation and experimental results which show the advantage and problems of using the nonlinear fluorescence effect in super resolution systems. The results show that it is almost impossible to get the nonlinear fluorescence effect without getting being limited by the fluorescence quenching, bleaching and saturation phenomena.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging IV
DOIs
StatePublished - 2011
EventSingle Molecule Spectroscopy and Imaging IV - San Francisco, CA, United States
Duration: 22 Jan 201123 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7905

Conference

ConferenceSingle Molecule Spectroscopy and Imaging IV
Country/TerritoryUnited States
CitySan Francisco, CA
Period22/01/1123/01/11

Keywords

  • Fluorescence
  • excitation power density
  • photo damage
  • saturated structured illumination
  • saturation
  • super resolution

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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