Superresolution optical fluctuation imaging (SOFI)

Thomas Dertinger; Ryan Colyer; Robert Vogel; Mike Heilemann; Markus Sauer; Jörg Enderlein; Shimon Weiss

doi:https://doi.org/10.1007/978-94-007-2555-3-2

Superresolution optical fluctuation imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Mike Heilemann, Markus Sauer, Jörg Enderlein, Shimon Weiss

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

Original language	English
Title of host publication	Nano-Biotechnology for Biomedical and Diagnostic Research
Editors	Eran Zahavy, Shmuel Yitzhaki, Arie Ordentlich, Avigdor Shafferman
Pages	17-21
Number of pages	5
DOIs	https://doi.org/10.1007/978-94-007-2555-3-2
State	Published - 2012
Externally published	Yes

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	733

Keywords

Correlation function
Statistical analysis
Superresolution

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

Access to Document

https://doi.org/10.1007/978-94-007-2555-3-2

Cite this

Dertinger, T., Colyer, R., Vogel, R., Heilemann, M., Sauer, M., Enderlein, J., & Weiss, S. (2012). Superresolution optical fluctuation imaging (SOFI). In E. Zahavy, S. Yitzhaki, A. Ordentlich, & A. Shafferman (Eds.), Nano-Biotechnology for Biomedical and Diagnostic Research (pp. 17-21). (Advances in Experimental Medicine and Biology; Vol. 733). https://doi.org/10.1007/978-94-007-2555-3-2

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title = "Superresolution optical fluctuation imaging (SOFI)",

abstract = "Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed {"}superresolution optical fluctuation imaging{"} (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.",

keywords = "Correlation function, Statistical analysis, Superresolution",

author = "Thomas Dertinger and Ryan Colyer and Robert Vogel and Mike Heilemann and Markus Sauer and J{\"o}rg Enderlein and Shimon Weiss",

note = "Funding Information: This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). J{\"o}rg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).",

year = "2012",

doi = "https://doi.org/10.1007/978-94-007-2555-3-2",

language = "الإنجليزيّة",

isbn = "9789400725546",

series = "Advances in Experimental Medicine and Biology",

pages = "17--21",

editor = "Eran Zahavy and Shmuel Yitzhaki and Arie Ordentlich and Avigdor Shafferman",

booktitle = "Nano-Biotechnology for Biomedical and Diagnostic Research",

}

Dertinger, T, Colyer, R, Vogel, R, Heilemann, M, Sauer, M, Enderlein, J & Weiss, S 2012, Superresolution optical fluctuation imaging (SOFI). in E Zahavy, S Yitzhaki, A Ordentlich & A Shafferman (eds), Nano-Biotechnology for Biomedical and Diagnostic Research. Advances in Experimental Medicine and Biology, vol. 733, pp. 17-21. https://doi.org/10.1007/978-94-007-2555-3-2

Superresolution optical fluctuation imaging (SOFI). / Dertinger, Thomas; Colyer, Ryan; Vogel, Robert et al.
Nano-Biotechnology for Biomedical and Diagnostic Research. ed. / Eran Zahavy; Shmuel Yitzhaki; Arie Ordentlich; Avigdor Shafferman. 2012. p. 17-21 (Advances in Experimental Medicine and Biology; Vol. 733).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Superresolution optical fluctuation imaging (SOFI)

AU - Dertinger, Thomas

AU - Colyer, Ryan

AU - Vogel, Robert

AU - Heilemann, Mike

AU - Sauer, Markus

AU - Enderlein, Jörg

AU - Weiss, Shimon

N1 - Funding Information: This work was supported by NIH grant# 5R01EB000312 and NIH grant# 1R01GM086197. Thomas Dertinger is supported by the German Science Foundation (DFG, fellowship # DE 1591/1 1). Jörg Enderlein acknowledges financial support by the Human Frontier Science Program (RGP46/2006) and by the German Federal Ministry of Education and Research (FKZ 13N9236).

PY - 2012

Y1 - 2012

N2 - Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

AB - Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

KW - Correlation function

KW - Statistical analysis

KW - Superresolution

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DO - https://doi.org/10.1007/978-94-007-2555-3-2

M3 - منشور من مؤتمر

C2 - 22101708

SN - 9789400725546

T3 - Advances in Experimental Medicine and Biology

SP - 17

EP - 21

BT - Nano-Biotechnology for Biomedical and Diagnostic Research

A2 - Zahavy, Eran

A2 - Yitzhaki, Shmuel

A2 - Ordentlich, Arie

A2 - Shafferman, Avigdor

ER -

Superresolution optical fluctuation imaging (SOFI)

Abstract

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Keywords

All Science Journal Classification (ASJC) codes

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