Abstract
We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy, achieving up to two-fold resolution increase as compared with a conventional CARS microscope. Phase-sensitivity is required for the standard pixel-reassignment procedure since the scattered field is coherent, thus the point-spread function is well-defined only for the field amplitude. We resolve the complex field by a simple add-on to the CARS setup enabling inline interferometry. Phase-sensitivity offers additional contrast which informs the spatial distribution of both resonant and nonresonant scatterers. As compared with alternative super-resolution schemes in coherent nonlinear microscopy, the proposed method is simple, requires only low-intensity excitation, and is compatible with any conventional forward-detected CARS imaging setup.
Original language | English |
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Article number | 10073 |
Journal | Nature Communications |
Volume | 15 |
DOIs | |
State | Published - 21 Nov 2024 |
All Science Journal Classification (ASJC) codes
- General Chemistry
- General Biochemistry,Genetics and Molecular Biology
- General Physics and Astronomy