1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

Klara R Mertinkus; J Tassilo Grün; Nadide Altincekic; Jasleen Kaur Bains; Betül Ceylan; Jan-Peter Ferner; Lucio Frydman; Boris Fürtig; Martin Hengesbach; Katharina F Hohmann; Daniel Hymon; Jihyun Kim; Božana Knezic; Mihajlo Novakovic; Andreas Oxenfarth; Stephen A Peter; Nusrat S Qureshi; Christian Richter; Tali Scherf; Andreas Schlundt; Robbin Schnieders; Harald Schwalbe; Elke Stirnal; Alexey Sudakov; Jennifer Vögele; Anna Wacker; Julia E Weigand; Julia Wirmer-Bartoschek; Maria A. Wirtz Martin; Jens Wöhnert

doi:10.1007/s12104-021-10053-4

¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

Klara R Mertinkus, J Tassilo Grün, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Jan-Peter Ferner, Lucio Frydman, Boris Fürtig, Martin Hengesbach, Katharina F Hohmann, Daniel Hymon, Jihyun Kim, Božana Knezic, Mihajlo Novakovic, Andreas Oxenfarth, Stephen A Peter, Nusrat S Qureshi, Christian Richter, Tali Scherf, Andreas SchlundtRobbin Schnieders, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Anna Wacker, Julia E Weigand, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Wöhnert

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5′- and 3′-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5′-untranslated region (5′-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5′-UUUCGU-3′ hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive 1H, 13C and 15N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.

Original language	English
Pages (from-to)	17-25
Number of pages	9
Journal	Biomolecular NMR Assignments
Volume	16
Issue number	1
DOIs	https://doi.org/10.1007/s12104-021-10053-4
State	Published - Apr 2022

All Science Journal Classification (ASJC) codes

Structural Biology
Biochemistry

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Mertinkus, K. R., Grün, J. T., Altincekic, N., Bains, J. K., Ceylan, B., Ferner, J.-P., Frydman, L., Fürtig, B., Hengesbach, M., Hohmann, K. F., Hymon, D., Kim, J., Knezic, B., Novakovic, M., Oxenfarth, A., Peter, S. A., Qureshi, N. S., Richter, C., Scherf, T., ... Wöhnert, J. (2022). ¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2. Biomolecular NMR Assignments, 16(1), 17-25. https://doi.org/10.1007/s12104-021-10053-4

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title = "1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2",

abstract = "The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5′- and 3′-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5′-untranslated region (5′-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5′-UUUCGU-3′ hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive 1H, 13C and 15N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.",

author = "Mertinkus, \{Klara R\} and Gr{\"u}n, \{J Tassilo\} and Nadide Altincekic and Bains, \{Jasleen Kaur\} and Bet{\"u}l Ceylan and Jan-Peter Ferner and Lucio Frydman and Boris F{\"u}rtig and Martin Hengesbach and Hohmann, \{Katharina F\} and Daniel Hymon and Jihyun Kim and Bo{\v z}ana Knezic and Mihajlo Novakovic and Andreas Oxenfarth and Peter, \{Stephen A\} and Qureshi, \{Nusrat S\} and Christian Richter and Tali Scherf and Andreas Schlundt and Robbin Schnieders and Harald Schwalbe and Elke Stirnal and Alexey Sudakov and Jennifer V{\"o}gele and Anna Wacker and Weigand, \{Julia E\} and Julia Wirmer-Bartoschek and \{Wirtz Martin\}, \{Maria A.\} and Jens W{\"o}hnert",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = apr,

doi = "10.1007/s12104-021-10053-4",

language = "الإنجليزيّة",

volume = "16",

pages = "17--25",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

Mertinkus, KR, Grün, JT, Altincekic, N, Bains, JK, Ceylan, B, Ferner, J-P, Frydman, L, Fürtig, B, Hengesbach, M, Hohmann, KF, Hymon, D, Kim, J, Knezic, B, Novakovic, M, Oxenfarth, A, Peter, SA, Qureshi, NS, Richter, C, Scherf, T, Schlundt, A, Schnieders, R, Schwalbe, H, Stirnal, E, Sudakov, A, Vögele, J, Wacker, A, Weigand, JE, Wirmer-Bartoschek, J, Wirtz Martin, MA & Wöhnert, J 2022, '¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2', Biomolecular NMR Assignments, vol. 16, no. 1, pp. 17-25. https://doi.org/10.1007/s12104-021-10053-4

TY - JOUR

T1 - 1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

AU - Mertinkus, Klara R

AU - Grün, J Tassilo

AU - Altincekic, Nadide

AU - Bains, Jasleen Kaur

AU - Ceylan, Betül

AU - Ferner, Jan-Peter

AU - Frydman, Lucio

AU - Fürtig, Boris

AU - Hengesbach, Martin

AU - Hohmann, Katharina F

AU - Hymon, Daniel

AU - Kim, Jihyun

AU - Knezic, Božana

AU - Novakovic, Mihajlo

AU - Oxenfarth, Andreas

AU - Peter, Stephen A

AU - Qureshi, Nusrat S

AU - Richter, Christian

AU - Scherf, Tali

AU - Schlundt, Andreas

AU - Schnieders, Robbin

AU - Schwalbe, Harald

AU - Stirnal, Elke

AU - Sudakov, Alexey

AU - Vögele, Jennifer

AU - Wacker, Anna

AU - Weigand, Julia E

AU - Wirmer-Bartoschek, Julia

AU - Wirtz Martin, Maria A.

AU - Wöhnert, Jens

PY - 2022/4

Y1 - 2022/4

N2 - The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5′- and 3′-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5′-untranslated region (5′-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5′-UUUCGU-3′ hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive 1H, 13C and 15N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.

AB - The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5′- and 3′-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5′-untranslated region (5′-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5′-UUUCGU-3′ hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive 1H, 13C and 15N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.

UR - http://www.scopus.com/inward/record.url?scp=85124762824&partnerID=8YFLogxK

U2 - 10.1007/s12104-021-10053-4

DO - 10.1007/s12104-021-10053-4

M3 - مقالة

C2 - 35178672

SN - 1874-2718

VL - 16

SP - 17

EP - 25

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 1

ER -

¹H, ¹³C and ¹⁵N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

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