Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding

Francesco Brunori; Deepak Kumar Padhi; Israel Alshanski; Joanna Freyse; Jan Niklas Dürig; Anja Penk; Luigi Vaccaro; Mattan Hurevich; Jörg Rademann; Shlomo Yitzchaik

doi:10.1002/cbic.202100552

Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding

Francesco Brunori, Deepak Kumar Padhi, Israel Alshanski, Joanna Freyse, Jan Niklas Dürig, Anja Penk, Luigi Vaccaro, Mattan Hurevich, Jörg Rademann, Shlomo Yitzchaik

Institute of Chemistry

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Cytokines such as interleukin-8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface-based glycan-protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe³⁺ on interleukin-8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine.

Original language	English
Article number	e202100552
Journal	ChemBioChem
Volume	23
Issue number	3
DOIs	https://doi.org/10.1002/cbic.202100552
State	Published - 4 Feb 2022

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.202100552

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title = "Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding",

abstract = "Cytokines such as interleukin-8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface-based glycan-protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe3+ on interleukin-8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine.",

author = "Francesco Brunori and Padhi, {Deepak Kumar} and Israel Alshanski and Joanna Freyse and D{\"u}rig, {Jan Niklas} and Anja Penk and Luigi Vaccaro and Mattan Hurevich and J{\"o}rg Rademann and Shlomo Yitzchaik",

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doi = "10.1002/cbic.202100552",

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TY - JOUR

T1 - Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding

AU - Brunori, Francesco

AU - Padhi, Deepak Kumar

AU - Alshanski, Israel

AU - Freyse, Joanna

AU - Dürig, Jan Niklas

AU - Penk, Anja

AU - Vaccaro, Luigi

AU - Hurevich, Mattan

AU - Rademann, Jörg

AU - Yitzchaik, Shlomo

PY - 2022/2/4

Y1 - 2022/2/4

N2 - Cytokines such as interleukin-8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface-based glycan-protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe3+ on interleukin-8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine.

AB - Cytokines such as interleukin-8 activate the immune system during infection and interact with sulfated glycosaminoglycans with specific sulfation patterns. In some cases, these interactions are mediated by metal ion binding which can be used to tune surface-based glycan-protein interactions. We evaluated the effect of both hyaluronan sulfation degree and Fe3+ on interleukin-8 binding by electrochemical impedance spectroscopy and surface characterizations. Our results show that sulfation degree and metal ion interactions have a synergistic effect in tuning the electrochemical response of the glycated surfaces to the cytokine.

UR - http://www.scopus.com/inward/record.url?scp=85120810353&partnerID=8YFLogxK

U2 - 10.1002/cbic.202100552

DO - 10.1002/cbic.202100552

M3 - مقالة

C2 - 34851004

SN - 1439-4227

VL - 23

JO - ChemBioChem

JF - ChemBioChem

IS - 3

M1 - e202100552

ER -

Sulfation Pattern Dependent Iron(III) Mediated Interleukin-8 Glycan Binding

Abstract

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