@inbook{d59f8339d2484320b5c55cd768ed1fa8,
title = "Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT",
abstract = "The ESCRT machinery mediates scission of the intercellular bridge that connects two daughter cells at the end of cytokinesis. Structured illumination microscopy (SIM) and cryo-soft-X-ray tomography (cryo-SXT) have been used in recent years to study the topology of ESCRT-driven cytokinetic abscission. These studies revealed that the intercellular bridge is occupied by cortical rings and spiral-like filaments and that ESCRTs form ring-like structures in this region during abscission. In this chapter, we provide two protocols: a protocol for determining the spatial organization of specific ESCRT components at the intercellular bridge using SIM and a protocol for resolving the ultrastructural organization of cortical filaments at the intercellular bridge using cryo-SXT.",
keywords = "Cell division, Correlative imaging, Cryo, Cytokinesis, ESCRT, ESCRT-III, Fluorescence imaging, Immunostaining, Membrane fission, Soft-X-ray, Super-resolution microscopy, Synchronization, Tomography",
author = "Shai Adar-Levor and Inna Goliand and Michael Elbaum and Natalie Elia",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
month = jan,
day = "1",
doi = "https://doi.org/10.1007/978-1-4939-9492-2_10",
language = "American English",
isbn = "978-1-4939-9491-5",
volume = "1998",
series = "Methods in Molecular Biology",
pages = "129--148",
editor = "Emmanuel Culetto and Renaud Legouis",
booktitle = "Methods in Molecular Biology",
}