Structures of Artificially Designed Discrete RNA Nanoarchitectures at Near-Atomic Resolution

Di Liu, Yaming Shao, Joseph A. Piccirilli, Yossi Weizmann

Research output: Contribution to journalArticlepeer-review


Although advances in nanotechnology have enabled the construction of complex and functional synthetic nucleic acid-based nanoarchitectures, high-resolution discrete structures are lacking because of the difficulty in obtaining good diffracting crystals. Here, we report the design and construction of RNA nanostructures based on homooligomerizable one-stranded tiles for x-ray crystallographic determination. We solved three structures to near-atomic resolution: a 2D parallelogram, a 3D nanobracelet unexpectedly formed from an RNA designed for a nanocage, and, eventually, a bona fide 3D nanocage designed with the guidance of the two previous structures. Structural details of their constituent motifs, such as kissing loops, branched kissing loops, and T-junctions, that resemble natural RNA motifs and resisted x-ray determination are revealed, providing insights into those natural motifs. This work unveils the largely unexplored potential of crystallography in gaining high-resolution feedback for nanoarchitectural design and suggests a route to investigate RNA motif structures by configuring them into nanoarchitectures.

Original languageAmerican English
Article numbereabf4459
Pages (from-to)1-14
JournalScience Advances
Issue number39
StatePublished - 22 Sep 2021

All Science Journal Classification (ASJC) codes

  • General


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