Structural characteristics that stabilize or destabilize different assembly levels of phycocyanin by urea

Ailie Marx, Noam Adir

Research output: Contribution to journalArticlepeer-review

Abstract

Phycocyanin is one of the two phycobiliproteins always found in the Phycobilisome antenna complex. It is always situated at the ends of the peripheral rods, adjacent to the core cylinders composed of allophycocyanin. The basic phycocyanin monomer is an (αβ) dimer of globin-like subunits with three covalently linked phycocyanobilin cofactors. Monomers assemble further into trimers, hexamers, and rods which include non-pigmented linker proteins. Upon isolation in low ionic strength solution, rods quickly disintegrate into phycocyanin trimers, which lose contacts with other phycobiliproteins and with the linker proteins. The trimers, however, are quite stable and only the presence of high concentrations of chaotropic agents (such as urea), very acidic solutions, or elevated temperatures induces monomerization, followed by separation between the subunits. We have recently determined the crystal structures of phycocyanin from the thremophilic cyanobacterium Thermosynechococcus vulcanus in the presence of 2 or 4 M urea, and shown that 4 M urea monomerizes the phycocyanin trimers. In this paper, we will describe the phycocyanin structures in 2 and 4 M urea more completely. By mapping out the urea positions, we describe the structural elements within the trimeric interaction interface that may be interrupted by the presence of 4 M urea. In addition, we also identify what are the structural characteristics that prevent 4 M urea from inducing subunit dissociation.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalPhotosynthesis Research
Volume121
Issue number1
DOIs
StatePublished - Jul 2014

Keywords

  • Complex assembly
  • Cyanobacteria
  • Photosynthesis
  • Protein denaturation
  • X-ray crystallography

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

Fingerprint

Dive into the research topics of 'Structural characteristics that stabilize or destabilize different assembly levels of phycocyanin by urea'. Together they form a unique fingerprint.

Cite this