TY - JOUR
T1 - Structural and functional differences between pheromonotropic and melanotropic PK/PBAN receptors
AU - Hariton-Shalev, Aliza
AU - Shalev, Moran
AU - Adir, Noam
AU - Belausov, Edurad
AU - Altstein, Miriam
N1 - Funding Information: This research was supported by the US–Israel Binational Agricultural Research and Development Fund (BARD) ( IS-4205-09 ) (MA). There is no conflict of interest in publishing the paper. This manuscript forms part of the Ph.D. thesis of Aliza Hariton-Shalev, a student at the Hebrew University of Jerusalem. This paper is contribution no. 507/12 from the Agricultural Research Organization, the Volcani Center, PO Box 6, Bet Dagan 50250, Israel.
PY - 2013
Y1 - 2013
N2 - Background The pyrokinin/pheromone biosynthesis-activating neuropeptide (PK/PBAN) plays a major role in regulating a wide range of physiological processes in insects. The ubiquitous and multifunctional nature of the PK/PBAN peptide family raises many questions regarding the mechanisms by which these neuropeptides elicit their effects and the nature of the receptors that mediate their functions. Methods A sex pheromone gland receptor of the PK/PBAN family from Heliothis peltigera female moth and a Spodoptera littoralis larval receptor were cloned and stably expressed, and their structural models, electrostatic potentials and cellular functional properties were evaluated. Results Homology modeling indicated highly conserved amino-acid residues in appropriate structural positions as experimentally shown for class A G-protein coupled receptors. Structural differences could be proposed and electrostatic potentials of the two receptor models revealed net charge differences. Calcium mobilization assays demonstrated that both receptors were fully functional and could initiate extracellular calcium influx to start PK/PBAN signal transduction. Evaluation of the signaling response of both receptors to PBAN and diapause hormone (DH) revealed a highly sensitive, though differential response. Both receptors responded to PBAN whereas only Spl-PK/PBAN-R exhibited a high response toward DH. Conclusions The structural, electrostatic and cellular functional differences indicate that different PK/PBAN in vivo functions may be mediated by different PK/PBAN receptors and elicited by different peptide(s). General significance The results advance our understanding of the mode of action of the PK/PBAN family, and might help in exploring novel high-affinity receptor-specific antagonists that can serve as a basis for the development of new families of insect-control agents.
AB - Background The pyrokinin/pheromone biosynthesis-activating neuropeptide (PK/PBAN) plays a major role in regulating a wide range of physiological processes in insects. The ubiquitous and multifunctional nature of the PK/PBAN peptide family raises many questions regarding the mechanisms by which these neuropeptides elicit their effects and the nature of the receptors that mediate their functions. Methods A sex pheromone gland receptor of the PK/PBAN family from Heliothis peltigera female moth and a Spodoptera littoralis larval receptor were cloned and stably expressed, and their structural models, electrostatic potentials and cellular functional properties were evaluated. Results Homology modeling indicated highly conserved amino-acid residues in appropriate structural positions as experimentally shown for class A G-protein coupled receptors. Structural differences could be proposed and electrostatic potentials of the two receptor models revealed net charge differences. Calcium mobilization assays demonstrated that both receptors were fully functional and could initiate extracellular calcium influx to start PK/PBAN signal transduction. Evaluation of the signaling response of both receptors to PBAN and diapause hormone (DH) revealed a highly sensitive, though differential response. Both receptors responded to PBAN whereas only Spl-PK/PBAN-R exhibited a high response toward DH. Conclusions The structural, electrostatic and cellular functional differences indicate that different PK/PBAN in vivo functions may be mediated by different PK/PBAN receptors and elicited by different peptide(s). General significance The results advance our understanding of the mode of action of the PK/PBAN family, and might help in exploring novel high-affinity receptor-specific antagonists that can serve as a basis for the development of new families of insect-control agents.
KW - Diapause hormone
KW - Heliothis peltigera
KW - Insect neuropeptide
KW - Pheromone biosynthesis-activating neuropeptide- receptor
KW - Spodoptera littoralis
UR - http://www.scopus.com/inward/record.url?scp=84881488326&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.bbagen.2013.06.041
DO - https://doi.org/10.1016/j.bbagen.2013.06.041
M3 - مقالة
SN - 0304-4165
VL - 1830
SP - 5036
EP - 5048
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 11
ER -