Steps Toward Enhancing the Fluorescence of Small-Molecule-Based Protein Labels Using Supramolecular Hosts

Pragati K Prasad; Leila Motiei; David Margulies

doi:10.1016/j.rechem.2021.100134

Steps Toward Enhancing the Fluorescence of Small-Molecule-Based Protein Labels Using Supramolecular Hosts

Pragati K Prasad
, Leila Motiei
, David Margulies

Department of Chemical and Structural Biology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

Original language	English GB
Article number	100134
Journal	Results in Chemistry
Volume	3
DOIs	https://doi.org/10.1016/j.rechem.2021.100134
State	Published - Jan 2021

ASJC Scopus subject areas

General Chemistry

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10.1016/j.rechem.2021.100134License: CC BY-NC-ND

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title = "Steps Toward Enhancing the Fluorescence of Small-Molecule-Based Protein Labels Using Supramolecular Hosts",

abstract = "Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.",

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year = "2021",

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doi = "10.1016/j.rechem.2021.100134",

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AU - Motiei, Leila

AU - Margulies, David

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Y1 - 2021/1

N2 - Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

AB - Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

UR - https://www.scopus.com/pages/publications/85105753463

U2 - 10.1016/j.rechem.2021.100134

DO - 10.1016/j.rechem.2021.100134

M3 - مقالة

SN - 2211-7156

VL - 3

JO - Results in Chemistry

JF - Results in Chemistry

M1 - 100134

ER -

Steps Toward Enhancing the Fluorescence of Small-Molecule-Based Protein Labels Using Supramolecular Hosts

Abstract

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