Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts

Pragati K Prasad; Leila Motiei; David Margulies

doi:https://doi.org/10.1016/j.rechem.2021.100134

Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts

Pragati K Prasad, Leila Motiei, David Margulies

Department of Chemical and Structural Biology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

Original language	English
Article number	100134
Number of pages	3
Journal	Results in Chemistry
Volume	3
DOIs	https://doi.org/10.1016/j.rechem.2021.100134
State	Published - Jan 2021

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title = "Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts",

abstract = "Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.",

author = "Prasad, {Pragati K} and Leila Motiei and David Margulies",

year = "2021",

month = jan,

doi = "https://doi.org/10.1016/j.rechem.2021.100134",

language = "الإنجليزيّة",

volume = "3",

journal = "Results in Chemistry",

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T1 - Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts

AU - Prasad, Pragati K

AU - Motiei, Leila

AU - Margulies, David

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N2 - Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

AB - Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

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U2 - https://doi.org/10.1016/j.rechem.2021.100134

DO - https://doi.org/10.1016/j.rechem.2021.100134

M3 - مقالة

SN - 2211-7156

VL - 3

JO - Results in Chemistry

JF - Results in Chemistry

M1 - 100134

ER -

Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts

Abstract

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