STEM Tomography in Biology

Sharon G. Wolf, Eyal Shimoni, Michael Elbaum, Lothar Houben

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

STEM modality provides major advantages for electron tomography of thicker (>300 nm) biological specimens, both for plastic-embedded, heavy-metal stained samples, and for vitrified, unstained cells. With the proliferation of modern TEM microscopes that allow for switching between TEM and STEM modes with relative ease, we expect the use of STEM tomography to increase. The concepts for STEM imaging are significantly different than for TEM, and therefore we will describe in detail the STEM imaging modality, followed by STEM tomography concepts and applications.
Original languageEnglish
Title of host publicationCellular Imaging
Subtitle of host publicationElectron Tomography and Related Techniques
EditorsEric Hanssen
Place of PublicationCham
PublisherSpringer Basel AG
Pages33-60
Number of pages28
ISBN (Electronic)9783319689975
ISBN (Print)9783319689951
DOIs
StatePublished - 2018

Publication series

NameBiological and Medical Physics, Biomedical Engineering

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