Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions

Tamar Azoulay-Shemer; Nikki Schwankl; Ido Rog; Menachem Moshelion; Julian I. Schroeder

doi:https://doi.org/10.1002/1873-3468.13198

Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO₂-regulated stomatal movements under short-day conditions

Tamar Azoulay-Shemer, Nikki Schwankl, Ido Rog, Menachem Moshelion, Julian I. Schroeder

The Hebrew University of Jerusalem, Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO₂ under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO₂] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO₂]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO₂-induced stomatal closing.

Original language	English
Pages (from-to)	2739-2759
Number of pages	21
Journal	FEBS Letters
Volume	592
Issue number	16
DOIs	https://doi.org/10.1002/1873-3468.13198
State	Published - Aug 2018

Keywords

Arabidopsis
gas exchange
guard cell
photoperiod
starch degradation
starch synthesis

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

https://doi.org/10.1002/1873-3468.13198

Cite this

@article{cde6cb693667481ba05e112df9426c35,

title = "Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions",

abstract = "Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.",

keywords = "Arabidopsis, gas exchange, guard cell, photoperiod, starch degradation, starch synthesis",

author = "Tamar Azoulay-Shemer and Nikki Schwankl and Ido Rog and Menachem Moshelion and Schroeder, {Julian I.}",

note = "Publisher Copyright: {\textcopyright} 2018 Federation of European Biochemical Societies",

year = "2018",

month = aug,

doi = "https://doi.org/10.1002/1873-3468.13198",

language = "الإنجليزيّة",

volume = "592",

pages = "2739--2759",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Starch biosynthesis by AGPase, but not starch degradation by BAM1/3 and SEX1, is rate-limiting for CO2-regulated stomatal movements under short-day conditions

AU - Azoulay-Shemer, Tamar

AU - Schwankl, Nikki

AU - Rog, Ido

AU - Moshelion, Menachem

AU - Schroeder, Julian I.

PY - 2018/8

Y1 - 2018/8

N2 - Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.

AB - Starch in guard cells functions in osmoregulation during stomatal movements. Starch metabolism is controlled by the circadian clock. We investigated the role of starch metabolism in stomatal responses to CO2 under different photoperiodic conditions. Guard cell starch levels correlate with low/high [CO2] exposure. Starch biosynthesis-deficient AGPase (ADG1) mutants but, unexpectedly, not the starch degradation-deficient BAM1, BAM3, and SEX1 mutants alone, are rate-limiting for stomatal conductance responses to [CO2]-shifts. Interestingly, AGPase is rate-limiting solely under short- but not long-day conditions. These findings suggest a model of enhanced AGPase activity in guard cells under short days such that starch biosynthesis becomes rate-limiting for CO2-induced stomatal closing.

KW - Arabidopsis

KW - gas exchange

KW - guard cell

KW - photoperiod

KW - starch degradation

KW - starch synthesis

UR - http://www.scopus.com/inward/record.url?scp=85052524900&partnerID=8YFLogxK

U2 - https://doi.org/10.1002/1873-3468.13198

DO - https://doi.org/10.1002/1873-3468.13198

M3 - مقالة

C2 - 30025149

SN - 0014-5793

VL - 592

SP - 2739

EP - 2759

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -