Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation

Ning Zhang, Yong Gang Chang, Roger Tseng, Sergey Ovchinnikov, Rakefet Schwarz, Andy LiWang

Research output: Contribution to journalArticlepeer-review

Abstract

Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four-stranded β-sheet that is flanked by four α-helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.

Original languageEnglish
Pages (from-to)2274-2280
Number of pages7
JournalProtein Science
Volume29
Issue number11
DOIs
StatePublished - 1 Nov 2020

Keywords

  • NMR spectroscopy
  • S. elongatus PCC 7942
  • biofilm
  • cyanobacteria
  • protein structure

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry

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