SNAP-Tag-Targeted MRI-Fluorescent Multimodal Probes

Ebaston Thankarajan, Shimrit Oz, Abed Saady, Kseniya Kulbitski, Mykhail O. Kompanets, Moris S. Eisen, Shai Berlin

Research output: Contribution to journalArticlepeer-review

Abstract

Magnetic resonance imaging (MRI) is a powerful imaging modality, widely employed in research and clinical settings. However, MRI images suffer from low signals and a lack of target specificity. We aimed to develop a multimodal imaging probe to detect targeted cells by MRI and fluorescence microscopy. We synthesized a trifunctional imaging probe consisting of a SNAP-tag substrate for irreversible and specific labelling of cells, cyanine dyes for bright fluorescence, and a chelated GdIII molecule for enhancing MRI contrast. Our probes exhibit specific and efficient labelling of genetically defined cells (expressing SNAP-tag at their membrane), bright fluorescence and MRI signal. Our synthetic approach provides a versatile platform for the production of multimodal imaging probes, particularly for light microscopy and MRI.

Original languageEnglish
Article numbere202300172
JournalChemBioChem
Volume24
Issue number16
Early online date24 Apr 2023
DOIs
StatePublished - 15 Aug 2023
Externally publishedYes

Keywords

  • MRI
  • SNAP-tag
  • contrast agents
  • fluorescent probes
  • imaging agents

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry

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