Skin regeneration with all accessory organs following ablation with irreversible electroporation

Alexander Golberg, Martin Villiger, G. Felix Broelsch, Kyle P. Quinn, Hassan Albadawi, Saiqa Khan, Michael T. Watkins, Irene Georgakoudi, William G. Austen, Marianna Bei, Brett E. Bouma, Martin C. Mihm, Martin L. Yarmush

Research output: Contribution to journalArticlepeer-review


Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500 V, with 70 μs pulse duration and 1000 pulses delivered at 3 Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing.

Original languageEnglish
Pages (from-to)98-113
Number of pages16
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number1
StatePublished - Jan 2018


  • electroporation
  • extracellular matrix
  • pulsed electric field
  • regeneration
  • scar
  • scarless
  • skin

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


Dive into the research topics of 'Skin regeneration with all accessory organs following ablation with irreversible electroporation'. Together they form a unique fingerprint.

Cite this