Abstract
Six-pack holography is adapted to reject out-of-focus objects in dynamic samples, using a single camera exposure and without any scanning. By illuminating the sample from six different angles in parallel using a low-coherence source, out-of-focus objects are laterally shifted in six different directions when projected onto the focal plane. Then pixel-wise averaging of the six reconstructed images creates a significantly clearer image, with rejection of out-of-focus objects. Dynamic imaging results are shown for swimming microalgae and flowing microbeads, including numerical refocusing by Fresnel propagation. The averaged images reduced the contribution of out-of-focus objects by up to 83% in comparison to standard holograms captured using the same light source, further improving the system sectioning capabilities. Both simulation and experimental results are presented.
Original language | English |
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Pages (from-to) | 632-646 |
Number of pages | 15 |
Journal | Optics Express |
Volume | 29 |
Issue number | 2 |
DOIs | |
State | Published - 18 Jan 2021 |
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics