Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells

Gopal Iyer, Jianmin Xu, Shimon Weiss

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs). The inherent photostable property of QDs can be exploited for understanding the arrangement and distribution of receptors in the plasma membrane. We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products. This protocol can be adapted universally for any type of QD structure with a coating of free amino groups.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages553-563
Number of pages11
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume751

Keywords

  • Antibodies
  • Cell membrane labeling
  • Epidermal growth factor receptor
  • Gel filtration
  • Live cell imaging
  • Peptides
  • Quantum dot

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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