Single-nucleus multiomic mapping of m6A methylomes and transcriptomes in native populations of cells with sn-m6A-CT

Kiyofumi Hamashima, Ka Wai Wong, Tsz Wing Sam, Jia Hao Jackie Teo, Reshma Taneja, Minh T.N. Le, Qi Jing Li, Jacob H. Hanna, Hu Li, Yuin Han Loh

Research output: Contribution to journalArticlepeer-review

Abstract

N6-methyladenosine (m6A) RNA modification plays important roles in the governance of gene expression and is temporally regulated in different cell states. In contrast to global m6A profiling in bulk sequencing, single-cell technologies for analyzing m6A heterogeneity are not extensively established. Here, we developed single-nucleus m6A-CUT&Tag (sn-m6A-CT) for simultaneous profiling of m6A methylomes and transcriptomes within a single nucleus using mouse embryonic stem cells (mESCs). m6A-CT is capable of enriching m6A-marked RNA molecules in situ, without isolating RNAs from cells. We adapted m6A-CT to the droplet-based single-cell omics platform and demonstrated high-throughput performance in analyzing nuclei isolated from thousands of cells from various cell types. We show that sn-m6A-CT profiling is sufficient to determine cell identity and allows the generation of cell-type-specific m6A methylome landscapes from heterogeneous populations. These indicate that sn-m6A-CT provides additional dimensions to multimodal datasets and insights into epitranscriptomic landscape in defining cell fate identity and states.

Original languageEnglish
Pages (from-to)3205-3216.e5
Number of pages18
JournalMolecular Cell
Volume83
Issue number17
DOIs
StatePublished - 7 Sep 2023

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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