Single-molecule nanosecond rotational diffusion analysis by time-correlated single-photon counting system

Dror Fixler, Lior Turgeman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In typical photon counting single-molecule polarization-sensitive experiments, the rotational correlation time of a single fluorescent molecule is much shorter than the temporal resolution of the detection system. Consequently, a large amount of incoming photons are lost during the integration time of photon counting experiment. In order to maximize the signal-tonoise ratio for a given temporal resolution of the system, this paper suggests examining the rate of convergence and the variance of the time-averaged measured fluorescence intensity. The new suggested method is able to extract the nanosecond rotational correlation times of fluorescein dye in the different viscosities of the medium.

Original languageEnglish
Title of host publicationImaging Systems and Applications, ISA 2013
DOIs
StatePublished - 2013
EventImaging Systems and Applications, ISA 2013 - Arlington, VA, United States
Duration: 23 Jun 201327 Jun 2013

Publication series

NameOptics InfoBase Conference Papers

Conference

ConferenceImaging Systems and Applications, ISA 2013
Country/TerritoryUnited States
CityArlington, VA
Period23/06/1327/06/13

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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