Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria

Camilla Ciolli Mattioli, Roi Avraham

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

In this chapter, we describe in detail how to perform a successful smFISH experiment and how to quantify mRNA transcripts in bacterial cells. The flexibility of the method allows for straightforward adaptation to different bacterial species and experimental conditions. Thanks to the feasibility of the approach, the method can easily be adapted by other laboratories. Finally, we believe that this method has a great potential to generate insights into the complicated life of bacteria.

Original languageEnglish
Title of host publicationFluorescence In Situ Hybridization (FISH)
Subtitle of host publicationMethods and Protocols
EditorsGal Haimovich
PublisherHumana Press
Chapter1
Pages3-23
Number of pages21
ISBN (Electronic)978-1-0716-3766-1
ISBN (Print)978-1-0716-3768-5
DOIs
StatePublished - 20 Mar 2024

Publication series

NameMethods in molecular biology (Clifton, N.J.)
Volume2784
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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