Single cell transcriptomics: Methods and applications

Itamar Kanter; Tomer Kalisky

doi:https://doi.org/10.3389/fonc.2015.00053

Single cell transcriptomics: Methods and applications

Itamar Kanter, Tomer Kalisky

Bar-Ilan University

Research output: Contribution to journal › Review article › peer-review

Abstract

Traditionally, gene expression measurements were performed on "bulk" samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular properties that were previously masked in "bulk" measurements can now be observed directly. In this review we will survey emerging technologies for single cell transcriptomics, and describe how they are used to study complex disease such as cancer, as well as other biological phenomena such as tissue regeneration, embryonic development, and immune response.

Original language	English
Article number	53
Journal	Frontiers in Oncology
Volume	5
Issue number	FEB
DOIs	https://doi.org/10.3389/fonc.2015.00053
State	Published - 2015

Keywords

FISH
Gene expression
RNA sequencing
Single cell
qPCR

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.3389/fonc.2015.00053

Cite this

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AU - Kalisky, Tomer

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AB - Traditionally, gene expression measurements were performed on "bulk" samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular properties that were previously masked in "bulk" measurements can now be observed directly. In this review we will survey emerging technologies for single cell transcriptomics, and describe how they are used to study complex disease such as cancer, as well as other biological phenomena such as tissue regeneration, embryonic development, and immune response.

KW - FISH

KW - Gene expression

KW - RNA sequencing

KW - Single cell

KW - qPCR

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M3 - مقالة مرجعية

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VL - 5

JO - Frontiers in Oncology

JF - Frontiers in Oncology

IS - FEB

M1 - 53

ER -

Single cell transcriptomics: Methods and applications

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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