@article{5d98e783e9d64ed6bba11a80e7256661,
title = "Single-cell transcriptome conservation in cryopreserved cells and tissues",
abstract = "A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.",
author = "Amy Guillaumet-Adkins and Gustavo Rodriguez-Esteban and Elisabetta Mereu and Maria Mendez-Lago and Jaitin, \{Diego A.\} and Alberto Villanueva and August Vidal and Alex Martinez-Marti and Enriqueta Felip and Ana Vivancos and Hadas Keren-Shaul and Simon Heath and Marta Gut and Ido Amit and Ivo Gut and Holger Heyn",
note = "We thank the cytometry unit of the CCiT (University of Barcelona) for successfully implementing single-cell sorting. Funding The research leading to these results received funding from the Olga Torres Foundation. AlV is supported by the Fondo de Investigaciones Sanitarias FIS (PI13-01339), Fundaci{\'o}n Mutua Madrile{\~n}a AP150932014 and the Asociaci{\'o}n Espa{\~n}ola Contra el C{\'a}ncer (AECC)-Barcelona. HH is a Miguel Servet (CP14/00229) researcher funded by the Spanish Institute of Health Carlos III (ISCIII). Core funding is from the ISCIII and the Generalitat de Catalunya. Authors{\textquoteright} contributions HH conceived and directed the study. AGA performed all experiments. AGA and MML implemented MARS-Seq sequencing library preparation protocols with the help from DAJ and IA. GRE and EM established single-cell RNA sequencing processing pipelines and performed statistical analysis with support of SH. AlV, AuV, AMM, EF, and AnV led the PDOX tumor models and contributed primary mouse samples. HH wrote the manuscript with support from MG and IG. All authors read and approved the final manuscript.",
year = "2017",
month = mar,
day = "1",
doi = "10.1186/s13059-017-1171-9",
language = "الإنجليزيّة",
volume = "18",
journal = "GENOME BIOLOGY",
issn = "1474-760X",
publisher = "BioMed Central Ltd.",
number = "1",
}