Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins

Changyang Linghu, I. Wen Chen, Dimitrii Tanese, Valeria Zampini, Or A. Shemesh

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Optogenetics allows control of neural activity in genetically targeted neuron populations by light. Optogenetic control of individual neurons in neural circuits would enable powerful, causal investigations of neural connectivity and function at single-cell level and provide insights into how neural circuits operate. Such single-cell resolution optogenetics in neuron populations requires precise sculpting of light and subcellular targeting of optogenetic molecules. Here we describe a group of methods for single-cell resolution optogenetics in neuron cultures, in mouse brain slices, and in mouse cortex in-vivo, via patterned light and soma-targeted optogenetic molecules.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages229-257
Number of pages29
DOIs
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2501

Keywords

  • Cell-body-targeted opsins
  • Computer-generated holography
  • Functional connectivity
  • Single-cell control
  • Single-cell optogenetics
  • Somatic optogenetics
  • Synaptic transmission
  • Two-photon stimulation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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