Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

Mingfeng Liao; Yang Liu; Jing Yuan; Yanling Wen; Gang Xu; Juanjuan Zhao; Lin Cheng; Jinxiu Li; Xin Wang; Fuxiang Wang; Lei Liu; Ido Amit; Shuye Zhang; Zheng Zhang

doi:10.1038/s41591-020-0901-9

Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

Mingfeng Liao, Yang Liu, Jing Yuan, Yanling Wen, Gang Xu, Juanjuan Zhao, Lin Cheng, Jinxiu Li, Xin Wang, Fuxiang Wang, Lei Liu, Ido Amit, Shuye Zhang, Zheng Zhang

Department of Systems Immunology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8(+) T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.

Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8(+) T cells in patients with moderate COVID-19.

Original language	English
Pages (from-to)	842-844
Number of pages	3
Journal	Nature Medicine
Volume	26
Issue number	6
Early online date	12 May 2020
DOIs	https://doi.org/10.1038/s41591-020-0901-9
State	Published - 1 Jun 2020

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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title = "Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19",

abstract = "Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8(+) T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8(+) T cells in patients with moderate COVID-19.",

author = "Mingfeng Liao and Yang Liu and Jing Yuan and Yanling Wen and Gang Xu and Juanjuan Zhao and Lin Cheng and Jinxiu Li and Xin Wang and Fuxiang Wang and Lei Liu and Ido Amit and Shuye Zhang and Zheng Zhang",

note = "We acknowledge the work and contribution of all the health providers from Shenzhen Third People{\textquoteright}s Hospital. This study was supported by the Science and Technology Innovation Committee of Shenzhen Municipality (202002073000002) and the National Natural Science Foundation of China (91442127 to Z.Z.; 81700540 to Y.L.). Z.Z., S.Z. and I.A. contributed to the study design and the manuscript preparation. J.Y., J.L., F.W. and L.L. contributed to the collection of clinical specimens. M.L., G.X. and X.W. performed scRNA-seq and TCR-seq capturing and library construction. J.Z. performed qRT–PCR. L.C. contributed to cytometric bead array detection. Y.L., Y.W. and I.A. contributed to data analysis. All authors approved the manuscript.",

year = "2020",

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doi = "10.1038/s41591-020-0901-9",

language = "الإنجليزيّة",

volume = "26",

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T1 - Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

AU - Liao, Mingfeng

AU - Liu, Yang

AU - Yuan, Jing

AU - Wen, Yanling

AU - Xu, Gang

AU - Zhao, Juanjuan

AU - Cheng, Lin

AU - Li, Jinxiu

AU - Wang, Xin

AU - Wang, Fuxiang

AU - Liu, Lei

AU - Amit, Ido

AU - Zhang, Shuye

AU - Zhang, Zheng

N1 - We acknowledge the work and contribution of all the health providers from Shenzhen Third People’s Hospital. This study was supported by the Science and Technology Innovation Committee of Shenzhen Municipality (202002073000002) and the National Natural Science Foundation of China (91442127 to Z.Z.; 81700540 to Y.L.). Z.Z., S.Z. and I.A. contributed to the study design and the manuscript preparation. J.Y., J.L., F.W. and L.L. contributed to the collection of clinical specimens. M.L., G.X. and X.W. performed scRNA-seq and TCR-seq capturing and library construction. J.Z. performed qRT–PCR. L.C. contributed to cytometric bead array detection. Y.L., Y.W. and I.A. contributed to data analysis. All authors approved the manuscript.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8(+) T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8(+) T cells in patients with moderate COVID-19.

AB - Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8(+) T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.Single-cell transcriptome and T cell receptor analysis of bronchoalveolar lavage fluid suggests enrichment of proinflammatory macrophages in patients with severe COVID-19 and the presence of clonally expanded CD8(+) T cells in patients with moderate COVID-19.

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DO - 10.1038/s41591-020-0901-9

M3 - مقالة

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JO - Nature Medicine

JF - Nature Medicine

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ER -

Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

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