Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq

Israel Antman, Ester Davis, Shahd Abu-Kamel, Merav Hecht, Yotam Drier

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We describe a protocol for H3K27ac ChIP paired-end sequencing and computational analysis of rearrangements. Our approach can be used to simultaneously map enhancers and their activity and to identify structural variations at enhancers. Since changes in enhancer activity and new enhancer translocations both play a major role in tumor initiation, progression, and response to therapy, this approach holds promise to uncover some of the mechanisms behind these processes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages131-140
Number of pages10
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2535

Keywords

  • ChIP-seq
  • Enhancer rearrangements
  • Histone modifications
  • Structural variation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

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