Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

Tal Laviv, Benjamin B. Kim, Jun Chu, Amy J. Lam, Michael Z. Lin, Ryohei Yasuda

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.

Original languageEnglish
Pages (from-to)989-992
Number of pages4
JournalNature Methods
Volume13
Issue number12
DOIs
StatePublished - 1 Dec 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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