Abstract
We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.
Original language | English |
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Pages (from-to) | 989-992 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 13 |
Issue number | 12 |
DOIs | |
State | Published - 1 Dec 2016 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology