TY - JOUR
T1 - Simple Continuous Glucose Monitoring in Freely Moving Mice
AU - Kleiman, Doron
AU - Littor, Mika
AU - Nawas, Mahmoud
AU - Ben-Haroush Schyr, Rachel
AU - Ben-Zvi, Danny
N1 - Funding Information: We thank Dvir Mintz DVM and the veterinary and husbandry staff in the animal facility, as well as members of our group, for fruitful discussions. This study was supported by an Israel Science Foundation grant 1541/21 awarded to D.B.Z. D.B.Z. is a Zuckerman STEM faculty. Publisher Copyright: © 2023 JoVE Journal of Visualized Experiments.
PY - 2023/2
Y1 - 2023/2
N2 - Mice are a common model organism used to study metabolic diseases such as diabetes mellitus. Glucose levels are typically measured by tail-bleeding, which requires handling the mice, causes stress, and does not provide data on freely behaving mice during the dark cycle. State-of-the-art continuous glucose measurement in mice requires inserting a probe into the aortic arch of the mouse, as well as a specialized telemetry system. This challenging and expensive method has not been adopted by most labs. Here, we present a simple protocol involving the utilization of commercially available continuous glucose monitors used by millions of patients to measure glucose continuously in mice as a part of basic research. The glucose-sensing probe is inserted into the subcutaneous space in the back of the mouse through a small incision to the skin and is held in place tightly using a couple of sutures. The device is sutured to the mouse skin to ensure it remains in place. The device can measure glucose levels for up to 2 weeks and sends the data to a nearby receiver without any need to handle the mice. Scripts for the basic data analysis of glucose levels recorded are provided. This method, from surgery to computational analysis, is cost-effective and potentially very useful in metabolic research.
AB - Mice are a common model organism used to study metabolic diseases such as diabetes mellitus. Glucose levels are typically measured by tail-bleeding, which requires handling the mice, causes stress, and does not provide data on freely behaving mice during the dark cycle. State-of-the-art continuous glucose measurement in mice requires inserting a probe into the aortic arch of the mouse, as well as a specialized telemetry system. This challenging and expensive method has not been adopted by most labs. Here, we present a simple protocol involving the utilization of commercially available continuous glucose monitors used by millions of patients to measure glucose continuously in mice as a part of basic research. The glucose-sensing probe is inserted into the subcutaneous space in the back of the mouse through a small incision to the skin and is held in place tightly using a couple of sutures. The device is sutured to the mouse skin to ensure it remains in place. The device can measure glucose levels for up to 2 weeks and sends the data to a nearby receiver without any need to handle the mice. Scripts for the basic data analysis of glucose levels recorded are provided. This method, from surgery to computational analysis, is cost-effective and potentially very useful in metabolic research.
UR - http://www.scopus.com/inward/record.url?scp=85150132732&partnerID=8YFLogxK
U2 - https://doi.org/10.3791/64743
DO - https://doi.org/10.3791/64743
M3 - Article
C2 - 36912552
SN - 1940-087X
VL - 2023
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 192
M1 - e64743
ER -