Short time behavior of fluorescence intensity fluctuations in single molecule polarization sensitive experiments

Lior Turgeman, Dror Fixler

Research output: Contribution to journalArticlepeer-review

Abstract

Recent developments in the field of single molecule orientation imaging have led us to devise a simple framework for analyzing fluorescence intensity fluctuations in single molecule polarization sensitive experiments. Based on the new framework, rotational dynamics of individual molecules are quantified, in this paper, from the short time behavior of the time averaged fluorescence intensity fluctuation trajectories. The suggested model can be applied in single molecule fluorescence fluctuations experiments to extract accurate expectation values of photon counts during very short integration time in which rotational diffusion is likely not to be averaged out.

Original languageEnglish
Pages (from-to)9276-9283
Number of pages8
JournalOptics Express
Volume20
Issue number8
DOIs
StatePublished - 9 Apr 2012

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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