TY - JOUR
T1 - Sequestration of a two-component response regulator by a riboswitch-regulated noncoding RNA
AU - Mellin, J. R.
AU - Koutero, Mikael
AU - Dar, Daniel
AU - Nahori, Marie Anne
AU - Sorek, Rotem
AU - Cossart, Pascale
PY - 2014/8/22
Y1 - 2014/8/22
N2 - Riboswitches are ligand-binding elements contained within the 5′ untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. Here, we show that in Listeria monocytogenes, a riboswitch that binds vitamin B12 controls expression of a noncoding regulatory RNA, Rli55. Rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require B12 as a cofactor. Defects in ethanolamine utilization, or in its regulation by Rli55, significantly attenuate Listeria virulence in mice. Rli55 functions by sequestering the two-component response regulator EutV by means of a EutV-binding site contained within the RNA. Thus, Rli55 is a riboswitch-regulated member of the small group of regulatory RNAs that function by sequestering a protein and reveals a distinctive mechanism of signal integration in bacterial gene regulation.
AB - Riboswitches are ligand-binding elements contained within the 5′ untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. Here, we show that in Listeria monocytogenes, a riboswitch that binds vitamin B12 controls expression of a noncoding regulatory RNA, Rli55. Rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require B12 as a cofactor. Defects in ethanolamine utilization, or in its regulation by Rli55, significantly attenuate Listeria virulence in mice. Rli55 functions by sequestering the two-component response regulator EutV by means of a EutV-binding site contained within the RNA. Thus, Rli55 is a riboswitch-regulated member of the small group of regulatory RNAs that function by sequestering a protein and reveals a distinctive mechanism of signal integration in bacterial gene regulation.
UR - http://www.scopus.com/inward/record.url?scp=84906674799&partnerID=8YFLogxK
U2 - https://doi.org/10.1126/science.1255083
DO - https://doi.org/10.1126/science.1255083
M3 - مقالة
SN - 0036-8075
VL - 345
SP - 940
EP - 943
JO - Science
JF - Science
IS - 6199
ER -