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Sequential allosteric mechanism of ATP hydrolysis by the CCT/TRiC chaperone is revealed through Arrhenius analysis

Research output: Contribution to journalArticlepeer-review

Abstract

Knowing the mechanism of allosteric switching is important for understanding how molecular machines work. The CCT/TRiC chaperonin nanomachine undergoes ATP-driven conformational changes that are crucial for its folding function. Here, we demonstrate that insight into its allosteric mechanism of ATP hydrolysis can be achieved by Arrhenius analysis. Our results show that ATP hydrolysis triggers sequential "conformational waves" They also suggest that these waves start from subunits CCT6 and CCT8 (or CCT3 and CCT6) and proceed clockwise and counterclockwise, respectively.

Original languageEnglish GB
Pages (from-to)5189-5194
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number20
DOIs
StatePublished - 16 May 2017

ASJC Scopus subject areas

  • General

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