Sensitive Cu2+-Cu2+ distance measurements in a protein-DNA complex by double-quantum coherence ESR

Sharon Ruthstein; Ming Ji; Preeti Mehta; Linda Jen-Jacobson; Sunil Saxena

doi:10.1021/jp4037149

Sensitive Cu²⁺-Cu²⁺ distance measurements in a protein-DNA complex by double-quantum coherence ESR

Sharon Ruthstein, Ming Ji, Preeti Mehta, Linda Jen-Jacobson, Sunil Saxena

Department of Chemistry

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

Double quantum coherence (DQC) ESR spectroscopy is applied to measure the Cu²⁺-Cu²⁺ distance in the EcoRI-DNA complex. A simple method is proposed to reduce the contribution of nuclear hyperfine and quadrupole interactions to such data. The effects of such interactions between the electron spin of Cu²⁺ and neighboring nuclei on the DQC data make it difficult to measure the nanometer range interspin distance. The DQC data is in good agreement with results obtained by double electron electron resonance (DEER) spectroscopy. At the same time, the signal-to-noise ratio per shot in DQC is high. Taken together, these results provide impetus for further development of paramagnetic metal ion-based DQC techniques.

Original language	English
Pages (from-to)	6227-6230
Number of pages	4
Journal	Journal of Physical Chemistry B
Volume	117
Issue number	20
DOIs	https://doi.org/10.1021/jp4037149
State	Published - 23 May 2013

All Science Journal Classification (ASJC) codes

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

Access to Document

10.1021/jp4037149

Cite this

@article{647bef3781684852979c84010f9ea488,

title = "Sensitive Cu2+-Cu2+ distance measurements in a protein-DNA complex by double-quantum coherence ESR",

abstract = "Double quantum coherence (DQC) ESR spectroscopy is applied to measure the Cu2+-Cu2+ distance in the EcoRI-DNA complex. A simple method is proposed to reduce the contribution of nuclear hyperfine and quadrupole interactions to such data. The effects of such interactions between the electron spin of Cu2+ and neighboring nuclei on the DQC data make it difficult to measure the nanometer range interspin distance. The DQC data is in good agreement with results obtained by double electron electron resonance (DEER) spectroscopy. At the same time, the signal-to-noise ratio per shot in DQC is high. Taken together, these results provide impetus for further development of paramagnetic metal ion-based DQC techniques.",

author = "Sharon Ruthstein and Ming Ji and Preeti Mehta and Linda Jen-Jacobson and Sunil Saxena",

year = "2013",

month = may,

day = "23",

doi = "10.1021/jp4037149",

language = "الإنجليزيّة",

volume = "117",

pages = "6227--6230",

journal = "Journal of Physical Chemistry B",

issn = "1520-6106",

publisher = "American Chemical Society",

number = "20",

}

TY - JOUR

T1 - Sensitive Cu2+-Cu2+ distance measurements in a protein-DNA complex by double-quantum coherence ESR

AU - Ruthstein, Sharon

AU - Ji, Ming

AU - Mehta, Preeti

AU - Jen-Jacobson, Linda

AU - Saxena, Sunil

PY - 2013/5/23

Y1 - 2013/5/23

N2 - Double quantum coherence (DQC) ESR spectroscopy is applied to measure the Cu2+-Cu2+ distance in the EcoRI-DNA complex. A simple method is proposed to reduce the contribution of nuclear hyperfine and quadrupole interactions to such data. The effects of such interactions between the electron spin of Cu2+ and neighboring nuclei on the DQC data make it difficult to measure the nanometer range interspin distance. The DQC data is in good agreement with results obtained by double electron electron resonance (DEER) spectroscopy. At the same time, the signal-to-noise ratio per shot in DQC is high. Taken together, these results provide impetus for further development of paramagnetic metal ion-based DQC techniques.

AB - Double quantum coherence (DQC) ESR spectroscopy is applied to measure the Cu2+-Cu2+ distance in the EcoRI-DNA complex. A simple method is proposed to reduce the contribution of nuclear hyperfine and quadrupole interactions to such data. The effects of such interactions between the electron spin of Cu2+ and neighboring nuclei on the DQC data make it difficult to measure the nanometer range interspin distance. The DQC data is in good agreement with results obtained by double electron electron resonance (DEER) spectroscopy. At the same time, the signal-to-noise ratio per shot in DQC is high. Taken together, these results provide impetus for further development of paramagnetic metal ion-based DQC techniques.

UR - http://www.scopus.com/inward/record.url?scp=84878072294&partnerID=8YFLogxK

U2 - 10.1021/jp4037149

DO - 10.1021/jp4037149

M3 - مقالة

C2 - 23631829

SN - 1520-6106

VL - 117

SP - 6227

EP - 6230

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 20

ER -

Sensitive Cu²⁺-Cu²⁺ distance measurements in a protein-DNA complex by double-quantum coherence ESR

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this