@inbook{90ed8bc2e6b94ec0acff0a694c7f600a,
title = "Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage",
abstract = "Posttranslational modifications of histone proteins regulate all biological processes requiring access to DNA. Monoubiquitination of histone H2B is a mark of actively transcribed genes in all eukaryotes that also plays a role in DNA replication and repair. Solution and structural studies of the mechanism by which histone ubiquitination modulates these processes depend on the ability to generate homogeneous preparations of nucleosomes containing ubiquitin conjugated to a specific lysine residue. We describe here methods for generating milligram quantities of histone H2B with ubiquitin (Ub) conjugated to Lys 120 via either a nonhydrolyzable, dichloroacetone linkage or a cleavable isopeptide bond. H2B-Ub with an isopeptide linkage is generated by a combination of intein-fusion protein derivatization and native chemical ligation, yielding a fully native ubiquitinated lysine that can be cleaved by Ub isopeptidases. We also describe how to reconstitute nucleosomes containing ubiquitinated H2B.",
keywords = "Chromatin, Histone modifications, Intein, Ubiquitin",
author = "Michael Morgan and Muhammad Jbara and Ashraf Brik and Cynthia Wolberger",
note = "Funding Information: This work was supported by National Institute of General Medical Sciences grant GM095822 (C.W.) and the US-Israel Binational Science Foundation (C.W. and A.B.). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jan,
day = "1",
doi = "10.1016/bs.mie.2019.01.003",
language = "English",
series = "Methods in Enzymology",
pages = "1--27",
booktitle = "Methods in Enzymology",
}