Self-assembly at the interface of λ-carrageenan and amphiphilic and cationic peptides: More than meets the eye

Gal Yosefi, Nitsan Eliraz, Sofiya Kolusheva, Hanna Rapaport, Ronit Bitton

Research output: Contribution to journalArticlepeer-review


Self-assembly of macroscopic membranes at the interface between self-assembling peptides and aqueous polymer solutions of opposite charge has been explored mostly due to the membranes' unique hierarchical structure of three distinct regions, including a layer of perpendicular fibers. We report here on the formation and characterization of self-assembled membranes made with λ-carrageenan and the cationic β-sheet peptides, Pro-Lys-(Phe-Lys)5-Pro (PFK). Using SAXS, SEM, ITC, and rheology, we compared these membranes' morphology and physical properties to membranes made with alginate. We recognized that the polysaccharide's single chain conformation, its solution's viscosity, the potential of hydrogen bonding and electrostatic interactions between the polysaccharides and the peptides charged groups, and the strength of these interactions all affect the properties of the resulting membranes. As a result, we identified that an interplay between the polymer-peptide strength of interactions and the stiffness of the polysaccharide's single chain could be used as a route to control the structure–function relationship of the membranes. These results provide valuable information for creating guidelines to design self-assembly membranes with specific properties.

Original languageAmerican English
Pages (from-to)1897-1907
Number of pages11
JournalJournal of Colloid and Interface Science
StatePublished - 15 Dec 2023


  • Carrageenan
  • Hydrogel
  • Peptide
  • Rheology
  • SAXS
  • Self-assembly

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Biomaterials
  • Colloid and Surface Chemistry


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