Selenium mitigates salt-induced oxidative stress in durum wheat (Triticum durum Desf.) seedlings by modulating chlorophyll fluorescence, osmolyte accumulation, and antioxidant system

Hydroponic experiments were conducted to investigate the effects of different concentrations of sodium selenate (Na₂SeO₄) and sodium selenite (Na₂SeO₃) on durum wheat seed germination and seedling growth under salt stress. The treatments used were 0 and 50 mM NaCl solutions, each supplemented with Na₂SeO₄ or Na₂SeO₃ at 0, 0.1, 1, 2, 4, 8, or 10 μM. Salt alone significantly inhibited seed germination and reduced seedling growth. Addition of low concentrations (0.1–4 μM) of Na₂SeO₄ or Na₂SeO₃ mitigated the adverse effects of salt stress on seed germination, biomass accumulation, and other physiological attributes. Among them, 1 μM Na₂SeO₄ was most effective at restoring seed germination rate, germination energy, and germination index, significantly increasing these parameters by about 12.35, 24.17, and 11.42%, respectively, compared to salt-stress conditions. Adding low concentrations of Na₂SeO₄ or Na₂SeO₃ to the salt solution also had positive effects on chlorophyll fluorescence indices, decreased the concentrations of free proline and malondialdehyde, as well as electrolyte leakage, and increased catalase, superoxide dismutase, and peroxidase activities in roots and shoots. However, high concentrations (8–10 μM) of Na₂SeO₄ or Na₂SeO₃ disrupted seed germination and seedling growth, with damage caused by Na₂SeO₃ being more severe than that by Na₂SeO₄. It is thus clear that exogenous selenium can improve the adaptability of processing wheat to salt stress and maintain higher photosynthetic rate by decreasing the accumulation of reactive oxygen species and alleviating the degree of membrane lipid peroxidation. Na₂SeO₄ was more effective than Na₂SeO₃ at all given concentrations.