Selective Lentiviral Mediated Targeting of Glia Cells in the Central Nervous System

M Fassler; I Weissberg; N Levi; A Monsonego; A Friedman; R Taube

doi:10.1007/s12031-012-9923-1

Selective Lentiviral Mediated Targeting of Glia Cells in the Central Nervous System

M Fassler, I Weissberg, N Levi, A Monsonego, A Friedman, R Taube

Ben-Gurion University of the Negev

Research output: Contribution to journal › Meeting Abstract

Abstract

Background: In a complex tissue of the central nervous system (CNS), cell cross-talk is essential to preserve normal functions. Current tools for dissecting the molecular mechanisms that mediate cell-cell interactions within the brain include molecular genetics, imaging and use of transgenic animals. However, these are technically challenging, time consuming and difficult to control. In this study we report the establishment and validation of a lentiviral-mediated gene-targeting platform to specific cells in the CNS. It combines unique features of self-inactivated lentiviruses that promote stable gene delivery into non-dividing cells and efficient display of single-chain variable region human fragments (scFv) or soluble IgG on the surface of viral particles.

Original language	American English
Pages (from-to)	S38-S38
Number of pages	1
Journal	Journal of Molecular Neuroscience
Volume	51
Issue number	Supplement 1
DOIs	https://doi.org/10.1007/s12031-012-9923-1
State	Published - Jan 2013

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title = "Selective Lentiviral Mediated Targeting of Glia Cells in the Central Nervous System",

abstract = "Background: In a complex tissue of the central nervous system (CNS), cell cross-talk is essential to preserve normal functions. Current tools for dissecting the molecular mechanisms that mediate cell-cell interactions within the brain include molecular genetics, imaging and use of transgenic animals. However, these are technically challenging, time consuming and difficult to control. In this study we report the establishment and validation of a lentiviral-mediated gene-targeting platform to specific cells in the CNS. It combines unique features of self-inactivated lentiviruses that promote stable gene delivery into non-dividing cells and efficient display of single-chain variable region human fragments (scFv) or soluble IgG on the surface of viral particles.",

author = "M Fassler and I Weissberg and N Levi and A Monsonego and A Friedman and R Taube",

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T1 - Selective Lentiviral Mediated Targeting of Glia Cells in the Central Nervous System

AU - Fassler, M

AU - Weissberg, I

AU - Levi, N

AU - Monsonego, A

AU - Friedman, A

AU - Taube, R

PY - 2013/1

Y1 - 2013/1

N2 - Background: In a complex tissue of the central nervous system (CNS), cell cross-talk is essential to preserve normal functions. Current tools for dissecting the molecular mechanisms that mediate cell-cell interactions within the brain include molecular genetics, imaging and use of transgenic animals. However, these are technically challenging, time consuming and difficult to control. In this study we report the establishment and validation of a lentiviral-mediated gene-targeting platform to specific cells in the CNS. It combines unique features of self-inactivated lentiviruses that promote stable gene delivery into non-dividing cells and efficient display of single-chain variable region human fragments (scFv) or soluble IgG on the surface of viral particles.

AB - Background: In a complex tissue of the central nervous system (CNS), cell cross-talk is essential to preserve normal functions. Current tools for dissecting the molecular mechanisms that mediate cell-cell interactions within the brain include molecular genetics, imaging and use of transgenic animals. However, these are technically challenging, time consuming and difficult to control. In this study we report the establishment and validation of a lentiviral-mediated gene-targeting platform to specific cells in the CNS. It combines unique features of self-inactivated lentiviruses that promote stable gene delivery into non-dividing cells and efficient display of single-chain variable region human fragments (scFv) or soluble IgG on the surface of viral particles.

U2 - 10.1007/s12031-012-9923-1

DO - 10.1007/s12031-012-9923-1

M3 - Meeting Abstract

SN - 0895-8696

VL - 51

SP - S38-S38

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

IS - Supplement 1

ER -

Selective Lentiviral Mediated Targeting of Glia Cells in the Central Nervous System

Abstract

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