SELECTIVE EXTRACTION OF BIOMOLECULES USING A BIDIRECTIONAL FLOW FILTER

Vesna Bacheva, Federico Paratore, Maya "bar Dolev", Baruch Rofman, Govind V. Kaigala, Moran Bercovici

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a novel microfluidic device for selective extraction of molecules based on their diffusivity. The device leverages alternated current field-effect electroosmosis (ac-FEEO) to create bidirectional flows, enabling both the separation of species, and the routing of low diffusivity species to an extraction outlet. The device can operate continuously and is suitable for processing sub-microliter volumes. The extraction efficiency of the system is more than 90% over tens of minutes, with a purity higher than 99.5%. We demonstrate the applicability of the system to the extraction of genomic DNA from short DNA fragments.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages23-24
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 10 Oct 202114 Oct 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2114/10/21

Keywords

  • DNA sorting
  • Diffusion
  • Electroosmotic Flow
  • Extraction
  • Separation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Fingerprint

Dive into the research topics of 'SELECTIVE EXTRACTION OF BIOMOLECULES USING A BIDIRECTIONAL FLOW FILTER'. Together they form a unique fingerprint.

Cite this