Scope and limitation of side-chain assisted ligation

Liat Spasser; K. S. Ajish Kumar; Ashraf Brik

doi:10.1002/psc.1336

Scope and limitation of side-chain assisted ligation

Liat Spasser, K. S. Ajish Kumar, Ashraf Brik

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

Original language	American English
Pages (from-to)	252-255
Number of pages	4
Journal	Journal of Peptide Science
Volume	17
Issue number	4
DOIs	https://doi.org/10.1002/psc.1336
State	Published - 1 Apr 2011

Keywords

Aminoacyl transfer
Native chemical ligation
Peptide ligation
Protein synthesis
Removable auxiliary

All Science Journal Classification (ASJC) codes

Structural Biology
Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Organic Chemistry

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10.1002/psc.1336

Cite this

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abstract = "Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.",

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AU - Brik, Ashraf

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AB - Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

KW - Aminoacyl transfer

KW - Native chemical ligation

KW - Peptide ligation

KW - Protein synthesis

KW - Removable auxiliary

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DO - 10.1002/psc.1336

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Scope and limitation of side-chain assisted ligation

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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