TY - JOUR
T1 - Sample preparation for mass spectrometry imaging of plant tissues
T2 - A review
AU - Dong, Yonghui
AU - Li, Bin
AU - Malitsky, Sergey
AU - Rogachev, Liana
AU - Aharoni, Asaph
AU - Kaftan, Filip
AU - Svatos, Ales
AU - Franceschi, Pietro
N1 - Tom and Sondra Rykoff Family Foundation Research; Israeli Centers of Research Excellence (i-CORE) program on Plant Adaptation to Changing Environment We thank the Tom and Sondra Rykoff Family Foundation Research and the Israeli Centers of Research Excellence (i-CORE) program on Plant Adaptation to Changing Environment for supporting the AA lab activity. AA is the incumbent of the Peter J. Cohn Professorial Chair.
PY - 2016/2/10
Y1 - 2016/2/10
N2 - Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining agents. These advantages make it an attractive molecular histology tool in medical, pharmaceutical, and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of MSI techniques in plants are reviewed according to different classes of plant metabolites.
AB - Mass spectrometry imaging (MSI) is a mass spectrometry based molecular ion imaging technique. It provides the means for ascertaining the spatial distribution of a large variety of analytes directly on tissue sample surfaces without any labeling or staining agents. These advantages make it an attractive molecular histology tool in medical, pharmaceutical, and biological research. Likewise, MSI has started gaining popularity in plant sciences; yet, information regarding sample preparation methods for plant tissues is still limited. Sample preparation is a crucial step that is directly associated with the quality and authenticity of the imaging results, it therefore demands in-depth studies based on the characteristics of plant samples. In this review, a sample preparation pipeline is discussed in detail and illustrated through selected practical examples. In particular, special concerns regarding sample preparation for plant imaging are critically evaluated. Finally, the applications of MSI techniques in plants are reviewed according to different classes of plant metabolites.
UR - http://www.scopus.com/inward/record.url?scp=84958546717&partnerID=8YFLogxK
U2 - https://doi.org/10.3389/fpls.2016.00060
DO - https://doi.org/10.3389/fpls.2016.00060
M3 - مقالة
SN - 1664-462X
VL - 7
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
IS - FEB2016
M1 - 60
ER -