Rrm3 and Pif1 division of labor during replication through leading and lagging strand G-quadruplex

Mor Varon, Daniel Dovrat, Jonathan Heuzé, Ioannis Tsirkas, Saurabh P. Singh, Philippe Pasero, Roberto Galletto, Amir Aharoni

Research output: Contribution to journalArticlepeer-review


Members of the conserved Pif1 family of 5'-3' DNA helicases can unwind G4s and mitigate their negative impact on genome stability. In Saccharomyces cerevisiae, two Pif1 family members, Pif1 and Rrm3, contribute to the suppression of genomic instability at diverse regions including telomeres, centromeres and tRNA genes. While Pif1 can resolve lagging strand G4s in vivo, little is known regarding Rrm3 function at G4s and its cooperation with Pif1 for G4 replication. Here, we monitored replication through G4 sequences in real time to show that Rrm3 is essential for efficient replisome progression through G4s located on the leading strand template, but not on the lagging strand. We found that Rrm3 importance for replication through G4s is dependent on its catalytic activity and its N-terminal unstructured region. Overall, we show that Rrm3 and Pif1 exhibit a division of labor that enables robust replication fork progression through leading and lagging strand G4s, respectively.

Original languageAmerican English
Pages (from-to)1753-1762
Number of pages10
JournalNucleic acids research
Issue number4
StatePublished - 28 Feb 2024

All Science Journal Classification (ASJC) codes

  • Genetics


Dive into the research topics of 'Rrm3 and Pif1 division of labor during replication through leading and lagging strand G-quadruplex'. Together they form a unique fingerprint.

Cite this