TY - JOUR
T1 - RNF20 and histone H2B ubiquitylation exert opposing effects in Basal-Like versus luminal breast cancer
AU - Tarcic, Ohad
AU - Granit, Roy Z
AU - Pateras, Ioannis S
AU - Masury, Hadas
AU - Maly, Bella
AU - Zwang, Yaara
AU - Yarden, Yosef
AU - Gorgoulis, Vassilis G
AU - Pikarsky, Eli
AU - Ben-Porath, Ittai
AU - Oren, Moshe
N1 - Publisher Copyright: © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Breast cancer subtypes display distinct biological traits that influence their clinical behavior and response to therapy. Recent studies have highlighted the importance of chromatin structure regulators in tumorigenesis. The RNF20-RNF40 E3 ubiquitin ligase complex monoubiquitylates histone H2B to generate H2Bub1, while the deubiquitinase (DUB) USP44 can remove this modification. We found that RNF20 and RNF40 expression and global H2Bub1 are relatively low, and USP44 expression is relatively high, in basal-like breast tumors compared with luminal tumors. Consistent with a tumor-suppressive role, silencing of RNF20 in basal-like breast cancer cells increased their proliferation and migration, and their tumorigenicity and metastatic capacity, partly through upregulation of inflammatory cytokines. In contrast, in luminal breast cancer cells, RNF20 silencing reduced proliferation, migration and tumorigenic and metastatic capacity, and compromised estrogen receptor transcriptional activity, indicating a tumor-promoting role. Notably, the effects of USP44 silencing on proliferation and migration in both cancer subtypes were opposite to those of RNF20 silencing. Hence, RNF20 and H2Bub1 have contrasting roles in distinct breast cancer subtypes, through differential regulation of key transcriptional programs underpinning the distinctive traits of each subtype.
AB - Breast cancer subtypes display distinct biological traits that influence their clinical behavior and response to therapy. Recent studies have highlighted the importance of chromatin structure regulators in tumorigenesis. The RNF20-RNF40 E3 ubiquitin ligase complex monoubiquitylates histone H2B to generate H2Bub1, while the deubiquitinase (DUB) USP44 can remove this modification. We found that RNF20 and RNF40 expression and global H2Bub1 are relatively low, and USP44 expression is relatively high, in basal-like breast tumors compared with luminal tumors. Consistent with a tumor-suppressive role, silencing of RNF20 in basal-like breast cancer cells increased their proliferation and migration, and their tumorigenicity and metastatic capacity, partly through upregulation of inflammatory cytokines. In contrast, in luminal breast cancer cells, RNF20 silencing reduced proliferation, migration and tumorigenic and metastatic capacity, and compromised estrogen receptor transcriptional activity, indicating a tumor-promoting role. Notably, the effects of USP44 silencing on proliferation and migration in both cancer subtypes were opposite to those of RNF20 silencing. Hence, RNF20 and H2Bub1 have contrasting roles in distinct breast cancer subtypes, through differential regulation of key transcriptional programs underpinning the distinctive traits of each subtype.
UR - http://www.scopus.com/inward/record.url?scp=85011579336&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/cdd.2016.126
DO - https://doi.org/10.1038/cdd.2016.126
M3 - مقالة
C2 - 28157208
SN - 1350-9047
VL - 24
SP - 694
EP - 704
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
IS - 4
ER -